An Extraction Method of Genome DNA from Gastrodia elata Scape for AFLP Analysis

1、

Objective: TO extract high-quality DNA from Chinese traditional medicine Gastrodia elata for AFLP and other molecular biological analysis.

目的：获得能用于中药天麻扩增片段长度多态性（AFLP）分析及其它分子生物学研究的高质量DNA。

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2、

We found ABP prolonged coagulation time ( CT) in mice, increased plasma prothrombin time ( PT) and kaolin partial thromboplastin time ( KPTT) in rats.

发现怀牛膝多糖ABP能延长小鼠凝血时间（CT）、大鼠血浆凝血酶原时间（PT）、白陶土部分凝血活酶时间（KPTT）。

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3、

The highest growth rate of hairy roots was observed on MS liquid medium ( shaked at 110r/ min at 25 ℃ in darkness), and their fresh weight was increased by 8.4 times after culturing for 25 days.

通过对液体培养条件的选择，发现牛膝毛状根在MS液体培养基上（25℃、110r/min暗培养）获得最大生长速率，经25d培养，牛膝毛状根鲜重增加了8.4倍。

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4、

Ribosomal rDNA ITS sequence analysis of root of Achyranthes bidentata

牛膝的rDNA ITS序列分析

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5、

Effects of Water Soluble CdTe QDs on Seed Germination of Achyranthes Bidentata

水溶性CdTe量子点对牛膝种子萌发的影响

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6、

The bioactive results suggest that hydroxyalkylated AbPS were able to inhibit the growth of these two kind of tumors, and improve the activity of NK cell of mice bearing Lewis lung cancer.

结果表明，不同取代度的羟烷基化牛膝多糖对Lewis肺癌和B16黑色素瘤均有明显的抑制作用，同时能明显促进Lewis肺癌小鼠的NK细胞活性。

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7、

Applied Mark RAPD analyzed 19 genetic diversity of Cyathula Officinalis.

应用RAPD标记分析了19个川牛膝种群的遗传多样性。

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8、

Methods The influence of isopsoralen and ecdysterone ( the active principles of Fructus Psoraleae, Radix Cyathulae) on the proliferation of ER α and ER β cells and on the expression levels of ER α and ER β mRNA by MTT assay and RT-PCR respectively.

方法采用MTT细胞增殖实验观察中药补骨脂、川牛膝的两种活性成分：异补骨脂素和蜕皮甾酮对ERα、ERβ阳性细胞T47D增殖的影响；

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9、

The optimal extraction process was selected with the orthogonal design, taking four main factors which effected the selection process: ethanol concentration, solvent volume, extraction time, extraction of the frequency of three levels of each factor to test.

本文采用正交设计对怀牛膝总皂苷提取方法进行了研究，选择乙醇浓度、溶剂量、提取时间以及次数作为影响ABS醇提取工艺的4个主要因素。每个因素3个水平进行试验。

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10、

By the methylation method and GC MS analysis, we studied the distribution of carboxymethyl groups in the sugar backbone of CM AbPS and conclude that the carboxymethyl groups mainly substitute on 4 position of the fructofuranose of the sugar backbone.

并通过样品甲基化和GC MS分析，对羧甲基牛膝多糖糖链中羧甲基的分布进行了研究，结果表明羧甲基牛膝多糖中羧甲基主要取代在糖链中呋喃果糖的4位上。

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11、

Study on HPLC Fingerprints Construction and Medicinal Materials Quality Evaluation of Cyathula Officinalis Kuan

川牛膝HPLC指纹图谱构建与药材质量评价研究

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12、

CONCLUSION: Niuxi Powder can markedly reduce rats'whole blood viscosity.

结论：牛膝散能显著降低大鼠全血粘度。

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13、

Results: Hela cell adherence assay data showed that the ABPS can obviously inhibit the adherence at the concentration of 0 8mg/ ml, the adherence rate was reduced from 257 0 ± 5 2 organisms/ cell to 63 6 ± 3 6 organisms/ cell.

结果：发现牛膝多糖浓度为0.8mg/ml时，对细菌的细胞粘附抑制最为明显，粘附率由（257.0±5.2）个细菌/细胞降低到（63.6±3.6）个细菌/细胞。

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14、

Extraction process: Drynaria, Epimedium, Achyranthes by alcohol extraction of active ingredients, with the L9 ( 34) orthogonal experiment to select the best extraction.

提取工艺：淫羊藿、骨碎补和牛膝采用醇提取有效成分，用L9（34）正交实验进行优选最佳提取工艺。

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15、

The results showed that relative standard deviations of the above-mentioned three methods were all less than 5% and the recoveries were 88.23%~ 102.98%. Most of the contents of different elements were significantly different among different drugs.

结果，以上三种方法的相对标准偏差（RSD）均小于5%，加标回收率范围在88.23%～102.98%之间，不同品种牛膝生药材的相应元素含量之间多数存在显著或极显著差异。

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16、

Bidentata. Methods To determine rDNA ITS base sequence by peculiar PCR technology.

方法利用特异性PCR技术对牛膝的rDNA ITS区碱基序列进行测定，报道了牛膝的rDNA ITS区的碱基序列。

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17、

The root of bidentate achyranthes formulating is completed under being that here theory guides, already in applying many years clinically, get fairly good clinical effect.

牛膝醇提物就是在此理论指导下完成的，已在临床应用多年，取得较好的临床效果。

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18、

The effect of Achyranthis polysaccharides on SOD and MDA in the rats'brain tissue with cerebral trauma

牛膝多糖对脑外伤大鼠脑组织中SOD与MDA影响的实验研究

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19、

OBJECTIVE To study the activated effects of Achyranthes bidentata polysaccharides ( ABPS) on human monocytes in vitro, inducing HLA-DR_(α) surface molecules expression of monocytes.

目的：研究牛膝根多糖（ABPS）在体外对单核细胞活性的调节及诱导单核细胞HLA-DRα表面分子的表达。

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20、

The results of Mankin score: The scores of model group and bidentata saponins group were significantly higher than the normal group ( P0.05).

Mankin评分结果：模型组、牛膝总皂甙组Mankin评分显著高于正常组（P0.05）。

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