transcriptase的例句大全

The replication of HBV DNA passes through reverse transcription. As the reverse transcriptase is lack of effective function of proofreading the bases, the HBV genome is easier to mutate than other DNA viruses.

HBV复制过程中要经过逆转录，由于逆转录酶缺乏有效的碱基校对功能，故HBV基因组比一般DNA病毒易发生变异。

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Research Progress in Targeting Human Telomerase Reverse Transcriptase ( hTERT) Promoter in Cancer Gene Therapy

靶向人端粒酶逆转录酶启动子在肿瘤基因治疗中的研究进展

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In the present study, reverse transcriptase polymerase chain reaction ( RT-PCR) was used to detect the changes of the mRNAs expression of 5-HT_ 2~ 7 receptor subtypes in the rat dorsal root ganglia ( DRG) induced by spared nerve injury ( SNI).

本研究利用反转录聚合酶链式反应（RT-PCR）方法，观察了坐骨神经分支选择性损伤（SNI）所致神经病理性痛条件下，大鼠背根神经节（DRG）中5-HT2~7受体亚型mRNAs的时程表达变化。

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Moreover, the detection limit had been improved by the use of Superscript reverse transcriptase.

并且，使用了Superscript反转录酶进行反转录，提高了检测的灵敏度。

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Expression of Human Telomerase Reverse Transcriptase and in Genitalia Leukoplakia and Squamous Cell Carcinoma of Vulva

人端粒酶逆转录酶在外阴白斑和外阴鳞癌中的表达

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Methods Gene expression associated with vascular channel in osteosarcoma endothelial cell was measured by semi-quantitative reverse transcriptase polymerase chain reaction using the transcriptional level of the house-keeping gene for internal calibration.

方法利用RT-PCR技术对骨肉瘤肿瘤细胞（MG63、U2OS）血管形成相关基因进行检测，并同内皮细胞（ECV304）相比较。

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Detection of Cucumber green mottle mosaic virus by real time Reverse Transcriptase Polymerase Chain Reaction

实时荧光RT-PCR方法检测黄瓜绿斑驳花叶病毒

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One step nested reverse transcriptase-polymerase chain reaction ( RT-PCR) was used.

使用一步法巢式逆转录聚合酶链扩增法（RT-PCR）。

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Forth, make inverse transcription parallel experiment with difference inverse transcriptase, it is easy to distinguish from false-positive process in RNA process in experiment, today, Northern blot is better method on false-positive of identification, some peoples who have result well by improved correctly.

4用两种不同的反转录酶进行反转录平行实验，很容易区分出RNA制备过程中造成的假阳性，目前假阳性鉴定大多还是以Northern印迹为基础，再作些适当的改进。

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Roles of the EnvZ/ OmpR two-component system in response to various stresses were investigated with mutational analysis, quantitative reverse transcriptase PCR ( qRT-PCR).

同时通过突变分析、定量逆转录聚合酶链反应（qRT-PCR）研究了EnvZ/OmpR二元系统在应对渗透压力中的作用。

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Extraction of total RNA from culture clones which were expanded, and then detecting the target gene was integrated into the genome of HeLa cells through reverse transcriptase PCR method ( RT-PCR).

对单克隆进行扩大培养，提取其总RNA，逆转录酶PCR法（Reverse transcriptase-PCR，RT-PCR）检测到目的基因已整合到HeLa细胞基因组中，说明重组质粒在细胞中稳定表达。

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Methods Total RNA in the metastatic lymph nodes from patients with colorectal cancer was extracted routinely, and reverse transcriptase PCR employed to amplify the heavy chain Fd and light chain κ cDNA.

方法取大肠癌病人转移淋巴结，提取淋巴结总RNA，逆转录PCR扩增重链Fd和K轻链cDNA。

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After sacrificing the rats in twelve weeks, serum and aorta were studied through the techniques: biochemistry, radioimmunoassay, immuno-histochemistry ( IHC), reverse transcriptase PCR ( RT-PCR) and western blot.

各组大鼠实验12周后处死，提取血清、主动脉、通过生化、放免、免疫组化、RT&PCR法及蛋白印迹杂交法进行研究。

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Objective To establish polymerase chain reaction ( PCR) and reverse transcriptase PCR ( RT-PCR) for detecting human cytomegalovirus ( HCMV) and to find out the relationship between diabetes mellitus ( DM) and HCMV infection.

目的建立检测人巨细胞病毒（HCMV）感染的聚合酶链反应/逆转录&聚合酶链反应（PCR/RT-PCR）方法，并探讨糖尿病与HCMV感染的关系。

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Method RhD mRNA was analyzed through reverse transcriptase PCR ( RT-PCR) and cDNA sequencing in 4 individuals with different Rh phenotypes ( CcDDEe 、 CCDDee 、 CcDDee and ccDdee).

方法采用逆转录PCR（RT PCR）技术检测4名不同Rh表型（CcDDEe、CCDDee、CcDDee和ccDdee）个体的RhD mRNA，然后进行cDNA测序分析。

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Methods R and/ or G banding technique was used for analysis of karyotype. DNA blotting for HRX gene rearrangement, and polymerase chain reaction ( PCR) and reverse transcriptase PCR ( RT PCR) for fusion gene detection.

方法用R带和（或）G带分带技术进行核型分析，DNA印迹法检测HRX重排，聚合酶链反应和逆转录聚合酶链反应进行融合基因检测。

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Differential Display of Asthma-Related Genes by Reverse-transcriptase PCR

采用差异显示法分离支气管哮喘病因多基因初探

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C fos gene expression level was semi quantified by reverse transcriptase ( RT) PCR.

采用逆转录（RT）PCR对C Fos基因表达水平进行半定量分析。

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Detection for HCV with FD thermostable reverse transcriptase mediated RT nested PCR

FD耐热逆转录酶介导的逆转录nested PCR检测丙型肝炎病毒

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