MScs

Results After induced by HGF and EGF for 7 days, the spindle shaped MSCs become round shaped and resembled hepatocyte-like cells.

结果骨髓间充质干细胞经HGF和EGF诱导7d后变为肝细胞样圆形；

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Cell cycles showed 90% cells of the third generation of MSCs were at G0/ G1 phase.

细胞周期显示，第3代MSCs约有90%的细胞处于G0/G1期。

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METHODS: After establishing stable culture methods for rat MSCs, they were induced by high level of myocardial extracts on day 3 of primary culture for 1 week.

方法：体外分离培养大鼠MSCS，检测纯度。于原代培养第3D加入自体心肌匀浆上清液持续作用1周。

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Multipotent adipose-derived MSCs were isolated from subcutaneous, ovarian, or circumrenal adipose tissue of rabbit according to our method. Briefly, minced adipose tissue were digested with 0.1% collagenase type I and 1% BSA for 90 min at 37 ℃ with intermittent shaking.

首次采用O.1%胶原酶型I+1%BSA，消化90min的分离方法，从家兔腹股沟皮下脂肪组织，卵巢脂肪组织，或肾脏周围脂肪组织中分离得到脂肪MSCs。

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BM-MSCs can be induced to chondrogenic differentiation by TGF-of β 3, insulin, sodium pyruvate, vitamin C and dexamethasone in vitro. 3.

骨髓间充质干细胞在体外经TGF-β3、胰岛素、丙酮酸钠、维生素C、地塞米松诱导后可向软骨方向诱导分化。

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BACKGROUND: Mesenchymal stem cells ( MSCs) can differentiate not only into inter stitial cells but also into parenchyma cell ( e.g.myocardial cells).

背景：骨髓间充质干细胞（Mesenchymal Stem cells，MSCs）不仅能分化成间质细胞，而且能向实质细胞（如心肌细胞）转化。

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It showed that the infection efficiency of MSCs achieved 98.38% while that of HSC only 13.55% at the MOI of 150.

实验结果表明，在MOI为150时，腺病毒感染MSC的效率达到98.38%，HSC为13.55%。

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BACKGROUND: Multipotential mesenchymal stem cells ( MSCs) can be differentiated into bone, fat, cartilage, muscle and endothelial cell at the specific conditions, so it draws great attentions of the related study.

背景：多潜能间充质干细胞在特定的培养条件下可以分化为骨、脂肪、软骨、肌肉以及内皮细胞，是目前倍受关注的一类具有多向分化潜能的组织干细胞。

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The flow cytometry showed 80% cells of the forth generation of MSCs were at G0/ G1 phase.

流式细胞仪检测显示，第4代MSCs约有80%处于G0/G1期。

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General morphological characteristics of MSCs were observed by cytochemical and immunofluorescent techniques.

采用细胞组织化学和免疫荧光技术观察MSCs的一般形态学特点；

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IDO enzyme activity in MSCs inhibited T-lymphocytes proliferation of MLR cultures.

MSCs的IDO活性能抑制MLC体系中 T 淋巴细胞增殖率.

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Methods MSCs were obtained from the tibia and femur bone marrow of SD rats and cultured by their adherence characteristics to culture flask.

方法利用SD大鼠间充质干细胞（取自股骨和胫骨）贴壁生长的特性，培养纯化，传代扩增。

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The 3rd-generation MSCs were cultured combining with PLGA-ASP-PEG in vitro, while cells cultured in PLGA as control group. The cell adhesion rate and the adhesive power were examined by conventional precipitation method and micropipette aspiration technique respectively.

将第3代MSCs以1.0×106/ml接种到PLGA-ASP-PEG材料上，测定细胞黏附力和黏附率；

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MSCs were distributing in regenerate tissue including alveolar bone, cementum, periodontal ligament.

MSCs分布在新生牙槽骨、新生结缔组织及新生牙骨质中。

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Conclusion MSCs are ideal seeded cells in bone tissue engineering.

结论大鼠骨髓间充质干细胞（MSCs）是骨组织工程中适宜的种子细胞。

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In addition to hematopoietic stem cells, there are also some mesenchymal stem cells ( MSCs) in bone marrow.

骨髓中除含有能分化发育成各种血细胞的造血干细胞之外，还含有可产生非造血组织的间充质干细胞（Mesenchymal Stem cells，MSCs）。

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MSCs were transfected in situ by plasmid DNA complexes, which were formed along degradation of multilayered structures in a sustained manner.

随着多层膜降解，质粒DNA复合物颗粒从多层膜中持续形成，进而原位转染骨髓间充质干细胞。

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Objective: To determine the effect of erythropoietin ( EP ) on the proliferation of mesenchymal stem cells ( MSCs ) in vitro.

目的: 观察促红细胞生成素 ( eryrthropoietin,EP ) 对大鼠骨髓间充质干细胞 ( mesenchymalstemcells,MSCs ) 增殖和细胞周期的影响.

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Conclusion: EP could enhance MSCs proliferation in dose-dependent manner.

结论: EP可以促进骨髓间充质干细胞增殖,并且这种影响呈剂量依赖性.

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Mesenchymal stem cells ( MSCs) is another kind of tissue stem cell following hematopoietic stem cell.

间充质干细胞（mesenchymal stem cell，MSC）是继造血干细胞之后近年研究较多的另一类组织干细胞。

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This is the address that other MSCs will use when sending messages to this MSC.

其他MSC在向这个MSC传送消息时,将使用到该地址.

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Bone marrow mesenchymal stem cells ( BM-MSCs ) are adult stem cells in bone marrow stroma.

骨髓间充质干细胞 ( BM-MSCs ) 是位于骨髓基质中的成体干细胞,有学者发现在特定条件下BM-MSCs可以生成多种细胞,如间充质细胞、成纤维细胞、成骨细胞、软骨细胞、脂肪细胞 、 肌肉细胞、上皮细胞、神经元和神经胶质细胞等.

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The plentiful and safe bone MSCs is an expected seed cell of cartilage tissue engineering.

骨髓间充质干细胞（MSCs）来源丰富、安全，是软骨组织工程的理想种子细胞；

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UCB-derived MSCs could support the proliferation and differentiation of UCB CD 34~ + cells in vitro.

脐血MSC与 CD34+细胞共掊养14d, CD34+细胞扩增率高于未共培养组4倍.

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Methods BM-MSCs mass was prepared by centrifugal in vitro.

方法体外培养扩增BM-MSCs,离心制备成细胞团.

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The bone from fresh cadaver donor was seeded with MSCs and cultured in rotary cell culture system for a week after being defated, decalcified, and sterilized, whose structure and cell on which were observed by scanning electron microscope.

新鲜尸体骨行脱脂、脱钙、灭菌制成同种异体部分脱钙骨，与成人骨髓MSC复合，在旋转细胞培养系统内培养IW，扫描电镜观察部分脱钙骨的结构，及细胞在材料表面的生长情况。

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Mesenchymal stem cells ( MSCs) can be isolated from various adult tissues of human by their ability of adhering plastic culture plate wall.

间充质干细胞（Mesenchymal Stem cells，MSCs）存在于成人体内许多组织内，可以通过其贴壁能力分离出来。

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Results: single injection of MSCs prevented the occurrence of severe, irreversible damage to bone and cartilage.

结果: 单次注射间充质干细胞可预防骨和软骨严重的不可逆性损伤.

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Objective: To investigate the character and multiplication of mesenchymal stem cells ( MSCs) from SD-rat bone marrow in vitro.

目的：探讨体外分离培养、纯化SD大鼠骨髓间充质干细胞（Mesenchymal Stem cells，MSCs）的方法，明确增殖及生长特征。

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Group D: Simple MSCs were cultured.

D组: 单纯MSCs培养.

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