cDNA

Gene Expression Changes after Heat Shock by cDNA Array Analysis

热休克诱导基因表达谱的变化

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Results The gloverin cDNA sequence designed artificially was expressed in COS 7 cells.

结果　人工设计的葛佬素cDNA序列在COS7中得到表达.

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The cDNA sequencing was performed to analyze the change of FV coding sequence by splice site mutation.

通过cDNA测序分析剪切位点突变引起编码序列的变化。

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Gene expression profile of rat mesenchymal stem cell spontaneous transformation determined by cDNA microarray analysis

芯片分析自发恶性转化的大鼠骨髓间充质干细胞的基因表达谱

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Objective: To clone latent infection membrane protein 2A ( LMP2A) cDNA of Epstein Barr virus and study its genetic engineering vaccine.

目的：克隆爱泼斯坦巴尔病毒（EBV）潜伏期膜蛋白2A（LMP2A）基因，进一步研究其基因工程疫苗。

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Objective : To clone the hTPO cDNA and study its expression level in COS? 7 cells.

目的: 克隆人血小板生成素(hTPO)cDNA,并研究其在COS - 7细胞中的表达.

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Use of Poliovirus cDNA 5 ′ NCR Together with Its Polymerase Gene Can Increase Foreign Gene Expression

利用脊髓灰质炎病毒5'NCR和RNA聚合酶基因可提高外源基因表达

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A large full-length gene bank has been contructured through large-scale cDNA cloning and sequencing.

本实验室大规模的cDNA克隆与测序工作积累了大量的全长基因库.

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The cDNA was subcloned into prokaryotic expression vector pET 3 d and overexpressed in E . coli BL 21 ( DE 3 ).

将该cDNA插入原核表达载体pET3d并在 大肠杆菌 BL21 ( DE3 ) 中过量表达.

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Methods Total RNA was extracted from peripheral blood lymphocytes ( PBL) of patients with glioma and subjected to reverse transcription PCR. Variable region genes ( Vk and VH gene) were amplified from the cDNA by using nested polymerase chain reaction.

方法从脑胶质瘤患者外周血淋巴细胞（PBL）中提取细胞总RNA，经逆转录后用套式多聚酶链反应（PCR）分别扩增抗体轻链VK和重链VH基因。

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CDNA cloning of a novel gene related to apoptosis by differential display RT PCR

用差别显示法寻找细胞凋亡的相关基因

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Selective detection of hepatitis B virus mRNA by random amplification cDNA ends

乙型肝炎病毒mRNA的选择性检测

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Conclusions The human leptin gene cDNA clone was constructed by RT PCR.

【结论】以逆转录聚合酶链反应方法成功地构建了人瘦素基因cDNA克隆。

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Photobiotin-labelled hantavirus cDNA probe prepared from recombinant M segment of R22 strain

光敏生物素标记流行性出血热病毒R22株M片段cDNA探针的研究

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CDNA-AFLP differential patterns for 5 selected genes were confirmed via real-time reverse transcription PCR analysis.

用荧光实时定量PCR分析法证实了cDNA-AFLP基因表达结果。

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Clone of EPO Gene from Human Fetal Liver cDNA by Reverse Transcription and PCR

人胎肝cDNA中EPO基因的克隆

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Objective To construct a cDNA expression library of Periplaneta Americana nymph.

目的　构建美洲大蠊若虫cDNA表达文库.

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Each plasmid contains a cDNA insert that was cloned via EcoRI and XhoI.

每个质体含有一段cDNA,此插入基因是利用EcoRI与XhoI来 复殖的.

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When GAM cDNA was transfected into COS cells, it appears as an 24 kDa expressing band.

将GAM分子cDNA转染COS细胞 后, 细胞裂解液电泳可见一分子量约为24×103的表达带,与预期的分子量相符合.

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Expression of the Tea cystatin cDNA in E.coli

茶树巯基蛋白酶抑制剂基因cDNA在大肠杆菌中的表达

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E . coli DH 5α was transformed by pUC 18 recombined with PRV CP gene cDNA.

香木瓜环斑病毒(PRV) 外壳蛋白(CP)基因cDNA克隆 到pUC18上构建成 大肠杆菌 (Escherickiacoli)表达载体.

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CDNA Cloning, Sequencing and Expression of Human nov Gene

人nov基因全长cDNA克隆、测序及其组织表达谱分析

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The GenBank accession number is AF 265294. The WVDE cDNA is 1, 746 bp long.

PCR法 得到小麦紫黄质脱环氧化酶(WVDE)的全系列,cDNA的 全长为1, 746.

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A high throughput in silico data analysis system for cDNA library, CLASP, was constructed by bioinformatic approaches.

应用生物信息学方法, 构建了一套针对cDNA或 EST文库 的高通量、自动化分析体系, CLASP(cDNALibraryAnalysisSystemPrimary).

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Comparison of the sensitivity between digoxigenin-and biotin-labelled cDNA probes

Dig和Biotin标记的流行性出血热病毒cDNA探针敏感性比较

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The homologue of monkey ESc-615 was cloned in rat by screening rat epididymis cDNA library.

为得到该基因在大鼠中的同源基因,采用了筛选大鼠附睾cDNA文库的方法,得到了猕猴附睾 ESc -615在大鼠附睾中的同源基因.

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Phylogenetic trees show that there are 64 MADS-box genes encoding different proteins, including 46 cDNA sequences.

结果表明,水稻中有64个MADS-box基因编码不同氨基酸, 其中已知cDNA序列的为43个.

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Objective : To clone and sequence cDNA of human lymphotoxin ( LT ) .

目的: 克隆人 淋巴毒素cDNA并测序.

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Cloning of human brevican cDNA and the expression of its mRNA in human Glioma.

人短蛋白聚糖cDNA的克隆及其mRNA在人脑胶质瘤中表达的研究.

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Identification of a Rice cDNA Encoding the Acyl CoA binding Protein ( ACBP )

一种水稻酰基辅酶A结合蛋白cDNA的鉴定 ( 英文 )

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