ribozyme

Gene Construction and Activity Detection of the Ribozyme Multi-targeting against Negative Strand of Potato Spindle Tuber Viroid in vitro

特异切割马铃薯纺锤形块茎类病毒负链的多价核酶的构建和体外活性测定

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Objective To detect activity of Giardia canis virus vector-mediated hammerhead ribozyme for KRR 1 in vitro transcript.

目的检测犬贾第虫病毒介导的锤头状核酶对犬贾第虫滋养体核仁功能性蛋白KRR1基因体外转录体切割效率.

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The genes of hammerhead ribozymes targeting against two and three sites on negative strand of potato spindle tuber viroid ( PSTVd) were designed, synthesized and cloned according to the action manner of hammerhead ribozyme.

根据锤头型核酶的作用模式，设计、合成并克隆了特异切割马铃薯纺锤形块茎类病毒（PSTVd）负链RNA不同区域位点的双价和三价锤头型核酶基因。

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Cleavage of negative strand RNA of potato leafroll virus replicase gene in vitro by ribozyme

特异切割马铃薯卷叶病毒复制酶基因负链的核酶研究

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Study on the Cleavage of Potato Leafroll Virus Coat Protein Gene in vitro by Ribozyme

应用核酶体外切割马铃薯卷叶病毒外壳蛋白基因的研究

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To design the G. Design Computer design of TIMP-1 ribozyme

切割瘢痕组织的TIMP-1核酶计算机辅助设计及体外表达载体的构建

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Methods Anti-NOX 4 ribozyme clones of mouse embryonic stem ( ES ) cells were differentiated into embryoid bodies ( EBs ).

方法用 核糖 酶技术获得尼克酰胺腺嘌呤二核苷酸磷酸氧化酶4低表达小鼠胚胎干细胞克隆,将小鼠胚胎干细胞分化为胚小体.

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Ribozyme Any RNA molecule that acts as an enzyme.

核酶:任何可以起到酶的作用的RNA分子.

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Objective : To confirm the cleavage activity of ribozyme Rz 199 to oncogene K ras messenger RNA.

目的: 确定人工设计的核酶Rz199对原癌基因K-rasmRNA的体外切割活性,为K-ras特异性核酶的体内应用提供依据.

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Conclusion The JWA ribozyme gene transfection could inhibit the expression of JWA gene and reduce the adhesion functions of BMSC.

结论JWA核酶基凶转染可抑制人BMSC内JWA基因的表达，抑制骨髓基质细胞黏附功能。

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Construction of the plasmid expressing synthesized Ribozyme gene for Marek's disease virus

马立克氏病病毒Ribozyme基因的合成克隆和表达质粒的构建

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Cloning and Transcription In Vitro of Mutated Ribozyme Cleaving the Negative Strand RNA of Potato Leafroll Virus Replicase Gene

针对马铃薯卷叶病毒复制酶基因负链的突变核酶的克隆及体外转录

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The CCR5 ribozyme molecule stops the patient's white blood cells producing CCR5, a protein that HIV uses to get into host cells.

这种CCR5核酶分子能中止了病人的白血细胞产生CCR5，后者是艾滋病毒用于进入宿主细胞的一种蛋白质。

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RESULTS: Ribozyme 145 decreased the expression of ET-1 mRNA in ECV 304 cells.

结果：设计的核酶R145降低ECV304细胞ET-1mRNA的表达。

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Northern blot analysis indicates that the 3 ′ terminal non-viral sequence including the ribozyme had been cleaved by the cis-hairpin ribozyme from the in vitro transcripts soon after the in vitro transcription reaction.

Northern杂交结果表明，包括核酶在内的3′末端非病毒序列在体外转录反应开始后就被顺式发夹核酶切除了。

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