plasmid

Via pollen-tube pathway the indica rice HD-357 was transformed with plasmid pTW-α containing Bar gene and PIN ⅱ gene.

利用花粉管通道法，用含抗除草剂基因bar及抗虫基因pin2的质粒pTW-α转化中籼HD357。

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Objective : To find a rapid method of screening positive recombinant plasmid.

目的: 建立一种快速筛选阳性重组质粒的方法.

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MSCs were transfected in situ by plasmid DNA complexes, which were formed along degradation of multilayered structures in a sustained manner.

随着多层膜降解，质粒DNA复合物颗粒从多层膜中持续形成，进而原位转染骨髓间充质干细胞。

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Comparative proteomics research on removing of large invasive plasmid pINV of Shigella flexneri 2a strain 2457T

驱除侵袭大质粒pINV对福氏2a志贺氏杆菌2457T影响的比较蛋白质组学研究

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The recombinant CYP 3 A 4 plasmid was successfully cloned and effectively expressed in Rosetta ( DE 3 ) 2 pLysS.

成功地使CYP3A4基因在原核系统中高效表达.

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A newly constructed αⅱ bA2334C GFP fusion protein expressing plasmid was used to determine its subcellular localization by laser confocal scanning microscopy.

为了明确突变亚基的亚细胞定位，构建了αⅡb基因A2334CGFP融合蛋白表达质粒并用激光共聚焦显微镜确定GFP融合蛋白的细胞内定位。

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But the electroporation efficiency of plasmid in ligation system is relatively low mainly caused by T4 DNA ligase.

连接体系中质粒的转化效率较纯质粒低，而T4DNA连接酶是影响其转化效率的主要因素。

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Lysosome and cellular plasmid are activated to restrain activity of heavy metals.

溶酶体和细胞质粒抑制重金属活性.

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Objective : To survey plasmid profiles ( PP ) of Neisseria gonorrhoeae in Nanchang.

目的: 研究南昌地区 淋病 奈瑟菌质粒 谱 型的分布情况.

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Objective To construct the plasmid expressing BDV p 40 gene.

目的构建博尔纳病病毒p40基因重组表达质粒.

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Objective To explore the elimination effect of radix Pulsatillae extracts on R plasmid of pseudomonas aeruginosa.

目的探讨中药白头翁提取液对铜绿假单胞菌R质粒的消除作用.

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F-20-7 is the inter-kingdom fusant of wine yeast 1450 ( Saccharomyces cerevisiae) and SD-2a ( Oenococcus oeni), which was obtained by College of Enology in 2003. Because the key enzyme genes are on the plasmid, so the ability of degrading malic acid is not stable.

F-20-7是西北农林科技大学葡萄酒学院2003年获得的以葡萄酒酵母（saccharomyces cerevisiae）1450和酒酒球菌（Oenococcus oeni）SD-2a为亲本的跨界融合子。

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However, plasmid-based gene transfer has been accompanied by remarkable biologic effects when used to mediate in vivo expression of potent secreted molecule, such as VEGF, so that the effects of gene transfer would be amplified effectively by a paracrine mechanism.

然而，质粒介导的转基因，如基因产物在体内是高效能的细胞分泌分子，如VEGF，可取得明显的生物学效应，主要因为基因转染的效能被细胞旁分泌作用扩大。

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The PNL-BDNF-IRES 2-EGFP plasmid was identified to be correct.

构建的慢病毒载体质粒PNL -BDNF-IRES2-EGFP 经SalI和 BamHI 双酶切鉴定正确,三质粒共转染293T细胞 后荧光激发可见大量绿色荧光.

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The recombinant leptin siRNAs plasmid vectors could be expressed in eukaryocyte, and then to evaluated them by using enzyme cutting and sequencing.

构建能在真核细胞中表达的靶向瘦素基因的siRNAs重组质粒，用酶切方法和测序法对重组体进行鉴定。

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Replication, transcription and origin of the two kinds of mitochondrial plasmid are introduced respectively.

还分别介绍了它们的复制机制 、 转录和起源.

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Based on the analysis of cytoplasmic like-plasmid, WB-a new male sterile cytoplasm identified recently by Huazhong Agricultural University was found to contain S_1 and S_2-type plasmids. The result indicated that WB was further confirmed belonging to S-group cytoplasmic male sterility.

通过线粒体类质粒分析，表明华中农业大学发现的新雄性不育胞质WB带有S组特有的S1、S2类质粒，据此将WB确认为S组雄性不育胞质。

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Conclusions The recombinant plasmid constructed is useful for establishing a transgenic mouse.

构建所得的重组质粒可用于下游的转基因工作.

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Objective Construct a recombinant plasmid pET 28 a-EDA-EDB , prepare the fusion EDA-EDB protein.

目的构建重组pET28a-EDA-EDB 质粒, 制备重组纤维连接蛋白EDA -EDB 融合蛋白.

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Figure 2 . Restriction pattern of the recombinant plasmid.

图2. 阳性重组子质粒酶切电泳图谱.

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Conclusion The recombinant plasmid was constructed successfully, and it actually can block the expression of TITIN.

结论pSITITIN具有确切阻断肌联蛋白表达的效果。

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Objective To investigate the enhancement effect of IL-4 expression plasmid on cathepsin B DNA vaccine of Schistosoma japonicum ( Sj) in mice.

目的探讨重组白细胞介素-4（IL-4）真核表达质粒增强日本血吸虫组织蛋白酶b DNA疫苗对小鼠的免疫保护效果。

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BVTech Plasmid is DNA plasmid drawing software free software.

画质粒图软件,可绘出用于发表文章的基因图.

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Objective To evaluate the therapeutic effect of VEGF plasmid in cerebral infarction of dog by MR-DWI.

目的　用MR -DWI 追踪VEGF质粒对家犬脑梗塞模型的治疗效果.

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Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.

方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。

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Structural Variation of Recombinant Plasmid DNA and It's Regulation on Transcription

质粒DNA的构型变化对转录的调控作用

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PC-3 cells were transfected with mGM-CSF expressing plasmid or the control vector by lipo-some transfection.

通过脂质体转染法,将含GM -CSF 基因的质粒及空质粒转染至PC -3 细胞.

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Effect of eukaryotic expression plasmid DNA methyltransferase gene on methylation status and transcription level of DNA mismatch repair genes in human colon cancer cell line

甲基转移酶1表达质粒对结肠癌细胞错配修复基因甲基化及其表达的影响

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Objective: To investigate the possibility of gene therapy with naked plasmid encoding interleukin 7 ( IL 7).

目的：探讨白细胞介素7裸DNA质粒用于小鼠肿瘤基因治疗的可行性。

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Results: The recombinant plasmid pUC 18 E 6 had been got.

结果: 获得重组质粒pUC18E6.

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